Events

Past events
















Dr. Ivano Legini from the Human Technopole gave an inspiring talk on their work combining high-resolution sequencing technologies - such as long-read sequencing, single-cell RNA sequencing and spatial transcriptomics - with stem cells-derived organoid models, where they control gene expression with optogenetics.


We were very happy to have prof. Barbara Treutlein from ETH Zurich D-​BSSE (from the scNL advisory board) present very exciting work on using single-cell technologies to understand brain development using organoids. At the end of the talk we also got a very intriguing discussion of brain regeneration in axolotls!


Interesting talk by Jessie van Buggenum from Radboud University, Nijmegen on "Integrated single-cell (phospho-)protein and RNA detection uncovers phenotypic characteristics of human antibody secreting cells". 


Exceptional event organized by the LBSP Foundation, that took place in person at the Plus Ultra Leiden Kadans. More information for this event can be found at: https://leidenbiosciencepark.nl/events/tech-talk-single-cell


Very exciting talks by Hagen Tilgner (Weill Cornell Medical College) and Susan Kloet on the use of long-read sequencing technologies. Susan kicked off with a comprehensive overview on upcoming and readily available long-read sequencing technologies suited for single-cell (which you can find HERE), after which Hagen presented his latest wor on the use of long-read sequencing technologies for profiling alternative RNA splice isoform usage across cell types in mouse and human brain. 


Ahmed Mahfouz and Anna Alemany, members of the scNL organizing committee, gave an overview of the hot papers on single-cell research for the Technology, Computation, and Application pilars. You can find our selection in THIS presentation.


We were very happy to host Mike Vaninsberghe (Hubrecht Institute) who presented his latest work on ribosome profiling with single cell resolution, and Gangqi Wang (LUMC) who showed us how he is using single cell metabolism in tissues (with spatial and cellular resolution!)


Four speakers gave excellent talks on a wide variety of single cell applications, ranging from intra-tumoral characterisation of T-cell states by Anne van der Leun (NKI), manipulation of stem cells by Micha Drukker (LACDR), characterising auto-antibodies in COVID-19 Monique van der Wijst (UMCG), and readouts of anti-cancer drug responses by Klaas Mulder (RIMLS).


We were very happy to host Bernd Bodenmiller (University of Zurich) who presented his latest work on highly multiplexed imaging of tissues with subcellular resolution by imaging mass cytometry.  Then Anna Alemany gave a great overview of spatial transcriptomics methods.


First, Ahmed Mahfouz discussed Clarke, Zoe A., et al. Nature protocols (2021). Next, Lieke Michielsen and Sara Cardoso shared with us their experiences benchmarking different cell annotation software and creating a colon cancer cell atlas. 


scNL brings together researchers working in different aspects of the single-cell field, such as Technology, Computation and Application. Short talks introduced each of these items by Stefan Semrau (A clusterability measure for single-cell RNA-seq data), Anna Alemany (Single-cell RNA sequencing beyond protein-coding genes) and Noel de Miranda (Deconstructing the complexity of anti-tumor immune responses in colorectal cancer with single-cell approaches).


A big thank you to all 140 participants who joined our first online meeting! The online parallel brainstorm sessions gave a nice start to find out what the needs and goals are for our expertise network. We celebrated our first event with a keynote lecture by Itay Yanai (New York University) with a great lecture on the use of single-cell sequencing and spatial transciptomics.